Exact(1)
Sample B, containing the culture medium with viable cells was treated as follows: cells treated with trypsin were centrifuged at 4,000 rpm for 15 min, the supernatant was eliminated and the pellet was treated with ZnSO4 as above described.
Similar(59)
The remaining cells were washed twice with culture medium and viable mononuclear cell number was determined by counting Trypan blue unstained cells in a hemocytometer.
As these metrics don't currently exist outside of retail sales, the data can make video games a viable advertising medium, with delineation of what's popular being the focus in deciding where ad dollars will go.
As shown in Figure 2a, after 24 h only 3% of neutrophils cultured with medium alone resulted viable.
In order to investigate the proliferation responses, serum-starved AoSMCs were incubated with viable P. gingivalis for 24 h (see above), whereafter the medium was replaced with medium containing 0.5% FBS for 24 h, 48 h and 72 h.
However, the use of Nile red has low sensitivity and poor reliability, when it is used with viable cells growing in a liquid culture medium and entrained in a FACS system (Lee et al. 2013).
Smaller size nuclear reactors (IAEA defines as "small" those reactors with power<300 MWe and "medium" with power<700 MWe) can represent a viable solution for both the stakeholders, especially for developing countries, or countries with not-highly-infrastructured and interconnected grids, or even for developed countries when limitation on capital at risk applies.
All spores were viable on YE medium, with a 2 2 segregation of the kanR marker.
Mammalian skin and ear biopsies stored in tissue culture medium with antibiotics at 4°C have produced viable cultures after 3 weeks (ML Houck, unpublished observation); tissue samples from birds, non-avian reptiles, and amphibians have also produced viable cell lines although storage time-tolerance is less than that for mammals (ML Houck, unpublished observation).
The pellet was resuspended in culture medium with 10% FCS, the amount of viable cells counted and plated with 300,000 alive cells in conventional 24-well plates and cultured at 37°C with 5% CO2.
Samples were periodically withdrawn and plated on rich medium for viable cell counts (Methods).
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