Sentence examples for medium with vehicle from inspiring English sources

The cultures were then washed twice and one culture received medium with 10 nM chemerin, another received medium with 100 nM chemerin21-157 and a third was added a medium with vehicle only as control.

Then, the cells were washed twice with phosphate-buffered saline (PBS) and cultured for 2 days in the oestrogen-deprived medium with vehicle (control), 1 n M E2, the indicated concentrations of gefitinib and/or fulvestrant.

Then, medium was replaced and cells were cultured in medium with vehicle (PBS) or medium with a combination of recombinant human IFN-γ (R&D systems, 50 ng/ml) and recombinant human IL-1β (R&D systems, 50 ng/ml).

Then, the cells were washed twice with PBS and cultured for 2 or 4 days in the oestrogen-deprived medium with vehicle (control), 10  μ M gefitinib, 1 n M E2, 1 n M E2 plus 100 n M fulvestrant, 1 n M E2 plus 10  μ M gefitinib or 1 n M E2 plus 100 n M fulvestrant and 10  μ M gefitinib.

Then, the cells were washed twice with PBS and cultured for 2 days in the oestrogen-deprived medium with vehicle (control), 10  μ M gefitinib, 1 n M E2, 1 n M E2 plus 100 n M fulvestrant, 1 n M E2 plus 10  μ M gefitinib or 1 n M E2 plus 100 n M fulvestrant and 10  μ M gefitinib.

The regular medium was replaced with OGD medium supplemented with vehicle or NaHS at 100 or 300 µM.

Cells were seeded at the concentration of 20000 cells/well in complete medium, treated with vehicle or the inhibitor as described above and after 48 hours they were fixed with 4% paraformaldehyde (Sigma-Aldrich, St.Louis, MO, USA) for 10 min followed by three rinses with PBS.

The culture medium with DMSO (vehicle) served as control.

Cells were then cultured for 24 hours in SCC12 medium with DMSO vehicle, 2 μM Y27632 or indicated concentrations of BDP5290 in an IncuCyte ZOOM (Essen Bioscience).

STHdhQ7/Q7 cells were transferred to the serum-free medium and incubated with vehicle (DMSO, 0.1 0.2% (v/v) final concentration) or THC, HU-210, Akti-1/2 and rapamycin for 24 h as described above.

Quiescent immortalized HMC kept for 24 hours in 0.5% serum-containing medium were incubated with vehicle, Escherichia coli LPS (100 μg/ml), glucose (30 mM), LPS + glucose, mannitol (30 mM), or LPS + mannitol.