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The human breast cell line MDA-MB-231 was obtained from American Type Culture Collection (ATCC, Manassas, VA, USA), and cells were grown in a 1 1 Ham F12 Medium and Dulbecco Modified Eagle Medium with supplements and 10% calf serum as described [ 18].
Cells were grown in yeast extract medium with supplements (YES) unless stated otherwise.
If nothing else stated, cells were stimulated in RPMI medium with supplements including 10% FCS.
Repeat this process two more times, and then resuspend the cell pellet in culture medium with supplements.
Zaprinast was prepared in dimethyl sulfoxide (DMSO; Sigma D2650) and diluted in R16 serum free culture medium with supplements.
Cultures were grown on RBM medium, with supplements, for 16 hours at 37°C, with 5% (v/v) CO2, before RNA extraction.
Similar(18)
Strains were grown on minimal solid or liquid medium with appropriate supplements (simplified as MM; [39]).
hESCs were cultured onto matrigel-coated plates in mTeSR1 medium with 5× supplement (Invitrogen).
HMEC-1 cells were maintained in Endothelial Basal Medium with Endothelial Supplement and 5% FCS.
Sand-cultured plants were salt-treated by replacing the modified Hoagland medium with medium supplemented with 200 mM NaCl or 300 mM mannitol.
Keratinocytes were maintained in Epilife medium supplemented with keratinocyte growth supplement.
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