Sentence examples for medium with subsequent from inspiring English sources

Thermomechanics of the flow through a granular medium with subsequent release of chemical compounds from the grains of solid fuel is addressed.

3 TBM was defined as CSF culture positivity for M. tuberculosis on Lowenstein Jensen medium with subsequent molecular confirmation (GenoType MTBDRplus version 2; Hain Lifescience, Nehren, Germany).

A 10−3 M stock solution was freshly prepared in the cell culture medium with subsequent serial dilutions from 10−3 to 10−7 M. AS (Angeli's salt) (Cayman) was used as HNO donor.

The HARV bioreactor minimizes mechanical stresses on cell aggregates in culture by producing a laminar flow of culture medium with subsequent low shear and turbulence in an optimized suspension culture.

As LIF has been found to exert its effects within 72 hours of exposure [ 30], LIF was removed from the medium following 3 days in culture by spinning cells and reseeding them into FGF2/N2 medium with subsequent culture to generate neurospheres.

Iron oxide-based magnetic particles are prepared by slowly adding an inorganic precursor of iron (III) chloride, dissolved in aqueous medium with the subsequent addition to the oily medium, followed by the addition of pH regulators [ 13– 17].

Then, the medium was changed to serum-free RPMI 1640 medium containing [H]serine with subsequent incubation with adenoviruses for an additional 18 h.

Chondrogenic differentiation was achieved through culturing of a pellet of 2.2×105 cells in differentiation medium for three weeks with subsequent assessment of acid mucopolysaccharides by 1% Alcian blue (Chroma, Köngen, Germany) for 10 30 min. [54].

To label endosomes the cells were incubated with FITC-dextran for 5 min, to label lysosomes they were incubated with FITC-dextran for 30 min followed by washing with serum-free medium and subsequent incubation with this medium for 90 min. After labelling of endosomes or lysosomes, the cells were washed with serum-free medium, and complete medium was added to the cells.

These treatments were grinding at a medium roughness level, or grinding with subsequent polishing for enhancing the surface integrity and finally, in all cases, micro-blasting by fine Al2O3 grains.

One of the methods used to monitor the development of geodynamic processes in seismically active zones is based on regular sounding of the geological medium by powerful seismic vibrators, with subsequent analysis of the time dynamics of the seismic field parameters.