Sentence examples for medium with stable from inspiring English sources

Proteins were adsorbed on MCD from 15% FBS solution (PAA) in McCoy's 5A medium with stable Glutamine without Phenolred (BioConcept).

Cells were grown in VLE-RPMI 1640 medium with stable glutamine (Biochrom, Berlin, Germany) containing 10% heat-inactivated FCS, 50 μM β-mercaptoethanol (Sigma, Germany), 100 U/ml penicillin and 100 μg/ml streptomycin sulfate (Invitrogen, Karlsruhe, Germany).

J774.1 cells were cultivated in complete Iscove's medium procured from Biochrom (Berlin, Germany) supplemented with 10% (v/v) FCS while DAUDI cells were grown in RPMI 1640 liquid medium with stable glutamine containing 20% (v/v) FCS.

A20 cells (BALB/c B cell lymphoma, H-2d), a gift of Professor K. Smith, and YAC-1 cells, were maintained in RPMI-1640 medium with stable Glutamine and supplemented with penicillin/streptomycin (all PAA), 10% FBS (Life technologies), and 50 μM β-mercaptoethanol (Sigma).

The mouse melanoma cell lines B16-F10 and B16-OVA (ATCC, Manassas, VA, USA) both derived from C57/BL6 mice were cultured in RPMI 1640 medium with stable glutamine (Biochrom, Berlin, Germany), supplemented with 10% heat-inactivated fetal bovine serum (Biochrom), 100 U/ml penicillin and 100  μg/ml streptomycin (Gibco, Carlsbad, CA, USA).

A20 (BALB/c, H-2d, B cell lymphoma) cells, a gift of Prof. K. Smith, were maintained in RPMI-1640 medium with stable glutamine and supplemented with penicillin/streptomycin (all PAA), 10% fetal bovine serum (Life Technologies), and 50 μM β-mercaptoethanol (Sigma).

T cells were cultured at 10 cells/ml, 37°C and 5% CO2 in RPMI 1640 medium supplemented with stable glutamine (Biochrom AG), 10% fetal calf serum (PAN Biotech) and 2 μg/ml ciprobay (Bayer).

Microcystis sp. RST 9501 was grown in Z8 medium replaced with stable isotope S-labeled MgSO4.7H2O (catalogue no. IS7080; 90 atom % S; Icon) for the detection and identification of sulfur-containing microcystins.

The cells were cultured at 10 cells/ml, 37°C and 5% CO2 in RPMI 1640 medium supplemented with stable glutamine (Biochrom AG), 10% fetal calf serum PAN Biotech), 2 μg/ml ciprobay (Bayer) and 50 μM β-mercaptoethanol (Sigma Aldrich).

RTCs were grown in complete liquid phase medium (RPMI 1640 with stable L-glutamine) supplemented with 10% heat-inactivated (30 min at 56°C) fetal bovine serum, 100 IU/mL penicillin, and 100  μg/mL streptomycin (Sigma).

Different cell populations are grown in medium containing amino acids labeled with stable light or heavy isotopes.