Sentence examples for medium with shaking from inspiring English sources

First, the cells were incubated overnight in SC medium with shaking (200 rpm, 30°C), and then they were collected and re-suspended by centrifugation (10,000×g, 20 s) in sterilized water.

Mutant strains that produced more β-glucan were obtained as follows: ATCC 31750 was grown in a 500 ml flask that contained 50 ml of YP medium with shaking at 30°C for 17 h.

The optimal conditions for cell growth were using Luria-Bertani (LB) as medium with shaking at 150 rpm, 37 °C, and pH 8.0 which had been confirmed by shift pH and temperature.

K. ohmeri strains were grown for 48 h on 2% xylose, and mixed sugars (2% xylose + 2% glucose) in minimal medium with shaking at 150 rpm at 30 °C.

M. tb H37Rv was cultured in Dubos medium containing 0.5% BSA, 0.75% Dextrose and 0.085% NaCl plus 0.15% Tween-80 (DTA medium) with shaking at 220 rpm at 37°C.

All of the experiments in this part were conducted in 300-ml Erlenmeyer flasks containing 100 ml of each medium with shaking at 100 rpm on a rotary shaker at 50°C.

Unless indicated otherwise, bacteria were routinely grown at 37°C in Luria-Bertani medium (LB) with shaking at 250 rpm.

Briefly, a single colony was inoculated in 50 ml YPD medium overnight with shaking at 150 rpm and 30°C.

E. coli strains were grown in Luria Bertani medium at 37°C with shaking (180 rpm) or on solid growth medium, which contained 1.6% (w/v) agar.

A single colony was cultured in LBA medium at 37°C with shaking at 200 rpm overnight, and inoculated subsequently into 1 liter of LBA medium.

If not stated otherwise, E. coli O157 H7 EDL933 (EHEC) (Collection de l'Institute Pasteur: CIP 106327) was incubated in liquid medium at 37°C with shaking (180 rpm) by adding 1 ml overnight culture (about 10 cfu) to 100 ml medium.