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As a positive control, we spiked Vero E6-conditioned medium with recombinant human IL29 (rhIL29) prior to concentration.
Control unsorted BM cells from mice that did not receive any human cells grew in medium with recombinant mouse growth factors but not in CFUs with human factors, while human UCB cells grew in both media (n = 2, each condition).
Prior to the run the platforms A3 and B3 are manually supplied with eight DWPs containing LB medium with recombinant bacteria.
Cells were exposed to TPL (IC20: 5.0 nM) with or without IDA (27.0 nM) for 24 h and then cultured in complete methylcellulose medium with recombinant cytokines for 14 days.
Finally, supplementation of the culture medium with recombinant Wnt4 stimulated in vitro proliferation capacity of basal myoepithelial and basal stem/progenitor cells from parous mice by +138% ± 22% and +140% ± 17%, respectively.
Alongside this analysis, it has been shown that when optic cups are grown from embryonic stem cells (ESCs) in culture, RPE development can be enhanced by supplementing the growth medium with recombinant Wnt ligands (Eiraku et al., 2011).
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Unsorted AGM cells (10·5 dpc) were cultured on a low-density monolayer of irradiated S17 stromal cells in serum-free medium alone or medium supplemented with recombinant BMP4 (10 ng/ml).
Every week the medium was replaced with fresh selection medium supplemented with recombinant interleukin-6 (100 U/ml).
Half the medium was replaced every 3 days with complete medium supplemented with recombinant IL-2 (50 IU/ml) (Roche).
The medium was washed with PBS and replaced with EBM-2 medium supplemented with recombinant bFGF (10 ng/ml) (Promega Corp).
Factor-dependent cells capable of sustained growth in liquid medium supplemented with recombinant IL3 were generated by serial replating.
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