Sentence examples for medium with nutrient from inspiring English sources

The collected cells were cultured in complete medium, consisting of Dulbecco's Modified Eagle's Medium with Nutrient Mixture F12 (DMEM/F12; GIBCO), 10% fetal bovine serum (GIBCO), and 1% penicillin/streptomycin (GIBCO).

Cell culture medium: Dulbecco's modified Eagle's medium with nutrient mixture F-12 (DMEM/F-12) and GlutaMAX-I™ supplemented with 10% heat-inactivated fetal bovine serum (FBS), penicillin/streptomycin (50 U/mL/50 µg/mL), fungizone (2.5 µg/mL), and human transferrin (5 µg/mL).

The human breast adenocarcinoma cell line MCF-7 was cultured in a mixed medium of Dulbecco's Modified Eagle Medium with nutrient mixture F12 (Invitrogen) lacking phenol red with 10% Foetal Bovine Serum (Gibco).

or Sigma (St . Louis MO, U.S.A .. Eagle's minimum essential media and DMEM/F12 [DMEM (Dulbecco's modified Eagle's medium) with nutrient F12 mixture in the ratio 1 1] were purchased from Mediatech/Life Technologies (Fisher Scientific).

It should be noted that due to a lack of nutrients in the DI water, the E. coli cells will eventually die if they are not resuspended in a medium with nutrients.

ReNCell VM cells were grown as an adherent monolayer on poly-ornithine(0.002%) laminin (2  μg/ml -coated tissue culture flasks in the presence of 20 ng/ml of human recombinant EGF and bFGF2 in DMEM:F12 medium with nutrients optimized for neural progenitor cell growth.

Some cells were grown in the same medium supplemented with Nutrient mixture F (Sigma # N6013) with no detectable advantages.

Human neuroblastoma SH-SY5Y cells were grown in Dulbecco's modified Eagle's medium supplemented with Nutrient mixture F-12 (Ham) (1 1, v/v) (Gibco, Gaithersburg, MD, USA), 2 g/L sodium bicarbonate (Sigma-Aldrich), 100 units/ml penicillin, 100 μg/ml streptomycin and 10%% fetal bovine serum (FBS).

MCF10AT1 cells were obtained from the Michigan Cancer Foundation and maintained in phenol red free Dulbecco's modified Eagle's medium with F12 nutrient mixtures (DMEM/F12; Gibco, BRL) and supplemented with 2.5% heat-treated equine serum, 20 ng/mL EGF, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C under 5% CO2 conditions.

Of the two components of cell history varied in our compendium past growth medium and the cell density reached in this medium, which can alter cell state even in log phase growth medium appeared to be better remembered by B. subtilis, with past density remembered best when originally grown in the medium richest with nutrients, LB.

The principle on which is based the test is the following: a standard number of spores is embedded in an agar medium with selected nutrients.