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a Phenotypic changes in rice plants after 30 days of growth in culture medium with normal Pi concentration (+P, control), Pi starvation (−P), and over-abundant Pi supply (++P).
N-deficiency was achieved by cultivation of the microalga in conical flasks containing 1 L. of culture medium with normal nitrogen concentration and three additional cultivations were run under N-deficient conditions (25, 50 and 75% of the original levels used in the control medium).
Slices were returned to culture medium with normal serum after each drug exposure.
After P14, a subpopulation of the cells was transferred to medium with normal glucose level (NG) (5 mM).
A several hour incubation in culture medium with normal Zn2+ fully restores responsiveness to the five active metals.
Briefly, strains were inoculated in 350 µL of SWM medium, with normal or zero copper, and incubated for 48 h at 30°C.
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An initial test on 100 transformants grown on low-dose 5-FOA medium plates identified 30 transformants which died when transferred to medium with normal-dose 5-FOA plates.
The negative control (nC) condition received DRG medium combined with normal SC medium.
By contrast, neither clodronate nor E2 stimulated MCF-7 cell growth in medium supplemented with normal, steroid-containing serum (complete medium).
Our results show that mRNA expression levels of SPCA1 are significantly increased in A7r5 cells cultured in high glucose (25.0 mM -supplemented mM -supplementedwith normediumucompared55 mM)-supplemented medium.
Following transfection with scrambled or IκBζ siRNA for 24 h, the medium was replaced with normal medium, and ATF3 -/- cells were treated with Kdo2-Lipid A. TNF-induced cell death was determined using a colorimetric 3-[4,5-dimetylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay.
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