Ham's F-12 medium with L-glutamine was purchased from Fisher Scientific (Logan, UT, USA).
Based on the results of the l-arginine addition experiments and after taking the cost into consideration, we found that supplementation of the medium with l-arginine was unsuitable for relieving the growth disturbance brought by arginine auxotroph.
Glutamine medium with l-glutamine (f.c. 1 or 10 mM) added to the basal medium was used to analyze the effect of glutamine on PP-V production and on intracellular ammonium level.
viciae, strain RU1809 did not grow on minimal medium with L-glutamate as the sole carbon and nitrogen source.
MDA-MB-468 cells were cultured in McCoy's 5A medium with L-glutamine (2mM) and 10% fetal bovine serum.
SL2 cells were grown at 26°C in Schneideŕs Drosophila medium with L-glutamine supplemented with 10% FBS, 100 U/ml penicilin and 100 µg/ml streptomycin.
HepG2, HL60, K526, LCLC-103H, SW403 and T47D cells were cultured in RPMI 1640 medium with L-Glutamine supplemented with 10% FCS.
HEK293 cells ATCC-CRL15733) were cultivated in RPMI medium with L-glutamine (PAA) supplemented with 10% heat-inactivated FCS under mentioned conditions.
The NIH 3T3 fibroblast cell line (American Type Culture Collection, Rockville, MD) was cultured in DMEM culture medium with L-glutamine (Invitrogen, Carlsbad, CA) supplemented with 10% fetal calf serum (Sigma-Aldridge) and 2% penicillin and streptomycin (Invitrogen).
KT71T cells growing in defined medium with L-malate synthesized photosynthetic pigments continuously in the light and darkness, but reached a much higher cell density in the light (Figure 5A).
The purified cells were then plated at a minimum of 2×105 cells/cm2 in 6-well plates and cultured under standard condition (5% CO2/balanced air) in Iscove's modified Dulbecco's medium with L-glutamine, 25 mM HEPES and 10% heat-inactivated fetal bovine serum.
