Sentence examples for medium with insulin from inspiring English sources

To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development.

HUVECs were harvested at the designated time points, and RNA was extracted and quantified for Microarray analysis (Affymetrix measurement) The medium with insulin was collected and stored at −20°C for the insulin detection (Insulin Myria, Technogenetics), while the medium without insulin was discharged.

Fresh medium with insulin was changed every 24 h.

Previous study showed that the PAMPS/PDMAAm DN gel significantly enhances chondrogenic differentiation of ATDC5 cells in a differentiation medium with insulin [ 1].

The INS-DEX-IBMX medium was replaced after 2 days to medium with insulin alone for another 2 days after which they were supplemented with 10% FBS every other day thereafter.

A basal medium was prepared by supplementing RPMI1640 medium with insulin, albumin, ferric citrate, ethanolamine, fatty acids, glutamine, sodium pyruvate, 2-mercaptoethanol, 1-thioglycerol, nonessential amino acids, and vitamins.

TM6 cells were grown in 100 mm dishes for 48 hours in Dulbecco's modified Eagle's medium (DMEM /F12 medium supplemented with insulin (5 μg/ml), epidermal growth factor (5 ng/ml), adult bovine serum (2%), and antibiotic antimycotic solution (1×) at 37°C in the presence of 5% CO2 in air [ 8].

Prior to dosing, the medium was replaced with preadipocyte medium supplemented with insulin (0.5 μg/mL).

Keratocytes, isolated from bovine and human corneas, cultured in serum-free medium supplemented with insulin, selenium and transferrin, assumed typical keratocyte morphology, converted to fibroblasts in serum-containing medium and reverted to keratocytes after serum-deprivation.

ESCs were differentiated into embryoid bodies in suspension culture in the presence of fetal bovine serum (Atlanta Biologicals, Lawrenceville, GA) and the absence of LIF, then grown in adherent culture using serum-free medium supplemented with insulin, transferrin, selenium (ITS, Gibco), and fibronectin (Sigma).

MCF-7 cells were cultured in the same medium supplemented with insulin (30 μg/ml; Sigma-Aldrich, St Louis, MO, USA).