Suggestions(2)
Exact(4)
Aliquots (2.5 µl) of diluted cultures were spotted onto YPDT40 solid medium with indicated pH values.
Slices were transferred to fresh 0.1% FBS-containing culture medium with indicated agents.
GNPs were cultured in medium with indicated reagents and BrdU was added 1 hr before the cells were fixed.
For hydrogen peroxide, we scored 5-h survival of adult day 5 fem-1 hc17) fem-1 hc17 S-banimalsdinm with indicated concentrations of hydrogen peroxide.
Similar(56)
For replacement cultivations, strains were pregrown for 24 h with glycerol as the carbon source, washed with sterile media without the carbon source, and transferred to new medium with the indicated carbon source.
Although the behaviour of a tumour in the body may not accord with their responses in vitro for many obvious reasons, we need to demonstrate that it does work as well in principle with enzymes as with deficient medium, already indicated in some previous reports (Storr and Burton, 1974; Terayama et al, 1982; Savoca et al, 1984; Miyazaki et al, 1990; Gong et al, 2000; Wheatley et al, 2002).
All cell lines were maintained in a basal medium as indicated, supplemented with 10% fetal bovine serum (FBS) and antibiotics, in a humid atmosphere of 5% CO2 and 95% air at 37 °C.
After seeding overnight, the cells were treated with a fresh medium as indicated.
For cytokine switching experiments, exponentially growing cells were washed 3 times in MEM and then plated at 2×105 cells/mL in culture medium with the cytokines indicated.
After 3 h, 1.2 ml of medium with or without indicated growth factors was added.
Using a robotic liquid handler (FreedomEvo, Tecan), 96-well plates were prepared with 150 μl of M9 minimal medium with sugars as indicated.
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