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Cells were treated for 30 min at 37 °C in growth medium with increasing concentrations ranging between 1 and 100 nM of either AT2S or SS14 (positive control).
Serial passage of seven S. aureus strains in medium with increasing concentrations of peptide resulted in an induced resistance at various levels in all strains.
Adaptation of a xylose-utilizing genetically engineered strain of Saccharomyces cerevisiae to sugarcane bagasse hydrolysates by cultivation during 353 h using medium with increasing concentrations of inhibitors, including phenolic compounds, furaldehydes and aliphatic acids, led to improved performance with respect to ethanol production.
Additionally the Im3m phase expels water to the surrounding aqueous medium with increasing temperature, while there is no significant change in the Pn3m structure.
Further experiments using C medium with increasing glucose concentrations from 10 to 70 mM, indicated that, in all sugar-dependent biofilm formers, maximum crystal violet staining intensity was reached in wells where bacteria were grown at 30 mM or higher glucose concentrations (data not shown).
After 15 minutes of incubation with parasites, which provides sufficient time for them to attach to cells, the free parasites were removed, and as dynasore activity is reversible after 20 minutes, the medium with increasing dynasore concentrations was added back until the end of the incubation period [18].
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The swelling of the gels in serum containing culture medium increased with increasing VPA content.
The viscosity of the medium increased with increasing additive content and the number of regenerated plantlets also increased.
To determine whether this TapA expression strategy could be applied to liquid fermentation, we cultured GS_P GAP -tapA in the liquid YPD medium supplemented with increasing concentrations of lovastatin in shake flask culture.
Briefly, endothelial cells were suspended in 0.1% CS medium supplemented with increasing concentrations of the test compounds and incubated at 37°C for 4 h.
They were then rinsed and grown in medium supplemented with increasing concentrations (from 0 to 10 m M) of each drug.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com