Cells were treated for 30 min at 37 °C in growth medium with increasing concentrations ranging between 1 and 100 nM of either AT2S or SS14 (positive control).
Serial passage of seven S. aureus strains in medium with increasing concentrations of peptide resulted in an induced resistance at various levels in all strains.
Adaptation of a xylose-utilizing genetically engineered strain of Saccharomyces cerevisiae to sugarcane bagasse hydrolysates by cultivation during 353 h using medium with increasing concentrations of inhibitors, including phenolic compounds, furaldehydes and aliphatic acids, led to improved performance with respect to ethanol production.
Additionally the Im3m phase expels water to the surrounding aqueous medium with increasing temperature, while there is no significant change in the Pn3m structure.
After 15 minutes of incubation with parasites, which provides sufficient time for them to attach to cells, the free parasites were removed, and as dynasore activity is reversible after 20 minutes, the medium with increasing dynasore concentrations was added back until the end of the incubation period [18].
Further experiments using C medium with increasing glucose concentrations from 10 to 70 mM, indicated that, in all sugar-dependent biofilm formers, maximum crystal violet staining intensity was reached in wells where bacteria were grown at 30 mM or higher glucose concentrations (data not shown).
For the experimental analysis of hysteresis, we grew PPK-KO carrying the tetracycline-inducible [23] ppk1 and rel-gfp in medium with increasing concentrations of tetracycline (inducer) and analyzed the distribution of cells expressing GFP by flow cytometry in the stationary phase (steady state) (Figure 6A, going up).
The uptake of NPs by the DCs was induced by incubation in cell culture medium with increasing concentrations of MNPs.
The supplementation of this chelex medium with increasing concentrations of ZnCl2 gradually restored a cell proliferation rate comparable to normal medium.
