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When supplemented with either 0.75 mM or 0.125 mM L-arginine, C. glutamicum ORN1 pHexA)(pVWEX1-exuT) grew in D-galacturonate and D-glucuronate minimal medium with growth rates of about 0.04 ± 0.01 h−1 (Table 3).
In this tissue-culture system, MLL-ENL acts as the class II mutation, whereas supplementation of the medium with growth factors presumably substitutes for a class I mutation.
Cells were then centrifuged and resuspended at a concentration of 105 cells/ml in StemSpan medium with growth factor cocktail for c-kit+ and CD34+ cells.
After 6/7 days of culture, neurospheres were collected by centrifugation, dissociated and kept in culture for additional 7 days by re-suspension in fresh DMEM-F12/Glutamax medium with growth factors (passage).
Moreover, these floating neurospheres can be maintained in undifferentiated proliferative state for at least 14-DIV (including 1 passage after 7-DIV) by culture in medium with growth factors (Figure 1-C).
The ventral horns were trypsinized in Hank"s solution for 13 minutes, the trypsin solution was replaced with motor neuron (MN) medium with growth factors and agitated to break up cell clumps.
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An immortalized normal prostate cell line (NPrEC) was maintained in Defined Keratinocyte-SFM medium with growth-promoting supplement (Invitrogen, Carlsbad, CA).
The conventional and most frequently used hepatogenic medium is basic medium supplemented with growth factors including epidermal growth factor, bFGF, HGF, nicotinamide, oncostatin M, dexamethasone and ITS premix.
The BTSCs were isolated from T98G and DB29 glioma cells, and cultured in neurobasal medium with epidermal growth factor+basic fibroblast growth factor.
The replacement of full medium enriched with growth factors and foetal calf serum with basal medium resulted in viable cells with similar gene expression profiles.
The expansion is normally made in tissue culture vessels in the presence of a culture medium supplemented with growth factors, serum, and other specific additives.
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