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Without further pH adjustment, the maximum hydrogen yield of 2.20 mol/mol glucose was obtained based on the optimized medium with further verified the practicability of this optimum strategy.
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A calculated amount of the second seed culture was centrifuged at 4 °C and 5000g for 10 min to harvest the cells, which were washed in the YNB medium with two further cycles of centrifugation (4 °C, 5000g).
Supplementation of the growth medium with glucose further enhanced CLC synthesis, which is consistent with the effect of glucose supplementation on cell surface carbohydrate content [41].
Viral inoculum was then replaced by fresh culture medium with no further renewal during the experiments.
The supplementation of the conditioned medium with EGF further optimized proliferation and mitochondrial activity of the cervical epithelial cells.
The cells were allowed to recover overnight in medium with serum before further treatments.
In its latest report, the International Atomic Energy Agency says the Iranians have amassed enough low- and medium-enriched uranium that, with further enrichment, could fuel as many as six nuclear weapons.
However, the International Atomic Energy Agency's latest report says the country has amassed a stockpile of low- and medium-enriched uranium that, with further enrichment, could fuel as many as six nuclear weapons.
While they use radiation from undulators in medium size electron storage rings with further wavelength purification by monochrometers, we propose to use direct radiation from a compact undulator.
Subsequently, cells were washed with cold 50 mM PBS, fresh culture medium with extracts added and further incubated for 48 h in humidified atmosphere of 5% CO2 at 37°C.
Pulsatile chonic treatment was carried out by incubating cultured cells with BMP7 for 15 h followed by replacing with fresh medium and further cultures of two days.
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