Sentence examples for medium with following from inspiring English sources

The cultures were grown in for 5 days at 28 °C with agitation at 200 rpm in 250 ml Erlenmeyer flasks, each containing 20 ml basal fermentation medium with following composition (g l−1) KH2PO4 1.5, NaCl 0.5, MgSO4·7H2O 0.2, Na2MoO4·2H2O 0.05, defatted soy bean meal 2, sucrose 20 and trace mineral solution 1 ml.

To test the effect of different carbon and nitrogen sources like glucose, lactose, yeast extract, peptone and beef extract on production of antimicrobial peptides, 0.5% of each of them added to minimal medium with following composition (g/l): Na2HPO4.2H2O, 7.9; KH2PO4, 3.0; NaCl, 0.5; NH4Cl, 1.0; pH 7.2.

>> -wrap-foot> This medium is based on Williams' E medium with the following supplements Shipping of hepatocytes using Hepacult cold storage solution can be carried out as follows: Prepare and label sterile cryovials.

Let us further consider a spherical crack source in a homogeneous medium with the following boundary conditions at r = R: u r e = Δ s, (57).

Mineral salt medium with the following composition was used: 2.0 g L-1 (NH4)2.1O4, 2.1 g L-1 KH2PO4, 0.2 g L-1 MgSO4 × 7 H2O, 0.1 g L-1 CaCl2 × 2 H2O, 0.006 g L-1 FeCl3 × 6 H2O, 0.1 ml L-1 of trace element solution SL6 (Schlegel et al.1961) and 30 g L-1 of sodium gluconate as carbon source.

The amoebae (2 × 10) were preincubated at 37°C for 30 min in medium with the following inhibitors: 1 100  μM NH4Cl, 0.5 10% (w/v) sucrose, 5 25  μM chlorpromazine, 5 10  μg/mL filipin, 25 100  μg/mL nystatin, 7.5 20 mM methyl- β-cyclodextrin, 100 300 nM wortmannin, 5  μM cytochalasin D, and 1-2  μM colchicine.

Mycelium samples for RT-qPCR analysis were obtained from a mycelium transfer experiment similar to the one described above, using minimal medium with the following carbon source compositions: 100 mM sorbitol (reference condition), glucose 5 mM, glucose 55 mM, fructose 5 mM, fructose 55 mM, mannose 5 mM and mannose 55 mM.

Among the isolates, 458 were identified as phenotypically resistant to at least one first-line anti-TB drug (isoniazid, rifampicin, ethambutol or streptomycin) by the proportion method on Lowenstein Jensen medium with the following concentrations: isoniazid (0.2 mg/L), rifampin (40 mg/L), ethambutol (2 mg/L) and streptomycin (4 mg/L).

Experiments analyzing the effect of etomoxir and UK5099 were performed on the XF-96 format using an assay medium with the following modifications: the glucose concentration was 5.5 mM, sodium pyruvate 0.15 mM, and palmitic acid was included at 100 μM conjugated to fatty-acid-free BSA as described in the Supplemental Information.

The preinoculum was prepared by transferring 1 mL of stock culture cells to 25 mL tubes containing 10 mL of sterile Man, Rogosa, and Sharpe (MRS, Oxoid) culture medium with the following composition: 20 g/L glucose, 10 g/L peptone, 8 g/L meat extract, 4 g/L yeast extract, 2 g/L triammonium citrate, 2 g/L K2HPO4, 5 g/L CH3COONa·3H2O, 0.2 g/L MgSO4·7H2O, and 0.05 g/L MnSO4·4H2O.

After 2 h at 37°C, 5% CO2, the complex was aspirated and washed 2 times with medium following replace by 1 ml of medium.