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In contrast, all four strains grew vigorously in defined medium with cellobiose.
Most importantly, ethanol production doubled during growth on minimal VM medium with cellobiose.
Cultures were transferred on cellobiose and then inoculated at 5% into two 400 ml bottles of LOD medium with cellobiose as sole carbon source.
This strain, when transformed by an expression vector containing an operon bglA I- bglT cloned from S. baltica OS155 gained the ability to grow on minimal medium with cellobiose as the sole carbon and energy source.
High-performance liquid chromatography (HPLC) analysis of fermentation products from Ccel_2485 mutant cells grown in defined VM medium with cellobiose showed that the lactate concentration decreased significantly to 0.09 ± 0.01 g/l, less than 10% of wild-type levels.
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The transformed yeast cells were grown on 2% agar YP medium plates with cellobiose, β-glycan, CMC, or PASC as the single carbon source.
Substantially higher transcription levels of all genes tested were detected when the wild-type strain was grown in a medium supplemented with cellobiose (C-CDM) as a sole carbon source (Table 2).
The experimental groups were the YPA medium with 2%% cellobiose and supplemented with an equal unit (2 units) of NpaBGS or Novo™ 188 β-glucosidase in the culture.
When P. glabrum was cultivated in medium with lactose, sucrose, cellobiose, Avicel, and CM-cellulose, enzyme activity was not detected.
fThe null mutant could grow in SC-URA medium with 1% cellobiose when the NpaBGS beta-glucosidase gene is functional inside.
Supernatant and cell pellet samples for metabolomic analysis were collected from duplicate stationary phase cultures grown in defined VM medium with 5.0 g/l cellobiose.
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