Sentence examples for medium with aeration from inspiring English sources
An overnight culture of bacteria was prepared for RNA extraction by diluting 1∶50 in 100 ml of fresh medium with aeration by rotary shaking (250 rpm).
All preselection cultures were grown to saturation at 37° in glycerol minimal medium with aeration by shaking.
A sample (50 μl) of the overnight culture was placed in fresh brain heart infusion medium with aeration and agitation and grown to the exponential phase.
Cells were grown in liquid Tris-acetic acid-phosphate (TAP) medium with aeration on a cycle of 12 hr of light and 12 hr of darkness.
Normal liquid cultures of all Synechocystis strains in this study were grown at 30°C in 500 mL shake flasks containing 300 mL BG11 medium with aeration by sterile air under constant illumination at a photosynthetic photon flux density of approximately 30 μmol photons m-2 s-1 of white light.
The Synechocystis sp. PCC6803 wild-type strain, the mutant strains Syn-XT14 with overexpression of the FAR gene, GQ6 with deletion of the slr1609 gene and GQ5 with overexpression of the slr1609 gene were respectively grown in a 500 mL Erlenmeyer flask containing 300 mL of BG11 medium with aeration by sterile air for fatty alkane or fatty alcohol analysis.
Inserts were suspended in a two litre basin of M-medium with aeration to generate water circulation.
The effect of aeration rate on P. purpureum growth, TFA content, and fatty acid yield was investigated in ASW culture medium, with the aeration rate range of 0.5 3 L/min.
All strains used were grown in LB liquid medium with high aeration.
Uniform plants were cultivated in black plastic boxes (15 plants per box) containing the culture medium with continual aeration; the volume of solution in each box was 1.5 L. The experiment was performed in a growth chamber under controlled conditions: 14 h illumination per day (5.00 a.m. to 7.00 p.m ., 800 μmolm-2 s-1 PPFD, with a 25 oC/18 oC day/night temperature and a relative humidity of 60%%.
Escherichia coli DH5α and BL-21 were grown in either Luria Bertani (LB) or 2xYT medium at 37°C with aeration.
