Sentence examples for medium with additives from inspiring English sources

The cell pellet was diluted with 1-mL medium and spread into a new cell culture flask containing 20 mL Ham's F-12 medium with additives.

Finally, Trypsin residues were removed with, first, centrifugation (1600 rpm/5 min) of the cell fluid (culture medium with additives and loose osteoblast cells) and, second, by subsequently adding 10 ml of culture medium.

For all in vitro experiments, the particles were dispersed by sonication at 1 mg/ml in culture medium with additives (described above) for 15 minutes, and then diluted to the concentrations to be used in the studies.

In this study we examined copper nanoparticles of various sizes obtained by the method of wire electric explosion in Ar-medium with additive of H2 (10 vol. %) at the pressure of 1.52·10 Pa, capacitive storage charging voltage −24 kV (the wire diameter 0.3 mm, length 75 mm).

Obtained results show that GA inhibits the Al corrosion in NaOH medium with potassium halide additives and inhibition efficiency increases with the increase in concentration and temperature.

Depending upon cell density, they were split and fresh culture medium with the corresponding additives replaced every 3 days.

Fibroblast cell lines were grown in α-MEM medium with the same additives at 37°C and 5% CO2 and were maintained at a low passage number.

Chromosomally encoded Hyd-5ΔTM−His was purified from the S. enterica LB03 (PT5, hydAΔTM−His) strain that had been cultured anaerobically in 10 litres of LB (low-salt) medium with no other additives.

The special technological medium for carburizing [12] with additives of a low-molecular polyethylene as a carbon content diffusing substance was used as a coolant.

The MIC determinations were performed by cultivating the two strains in BHI medium on Bioscreen plates with additives and measuring optical densities, i.e. growth, of the cultures (see Materials and Methods for details).

Cells were repropagated in three-dimensional lrECM cultures for five days and the medium was replaced by (D MEM/F12 supplemented with additives (20 ng/ml EGF; 10 μg/ml insulin; 0.5 μg/ml hydrocortisone; 100 ng/ml cholera toxin).