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Therefore, in the present study, BM-hMSCs were cultivated in serum free growth medium or serum free growth medium with additional 4 or 6 mM Ca2+ for 3 weeks, following which, the proliferation and osteoblastic differentiation of these cells were evaluated.
The best lipid producer among the three new isolates was CPOH4 with 34.6% lipid per dry biomass and can therefore be classified as oleaginous under the chosen conditions in YM medium with additional glucose and shake flasks.
However, SSOH12 yielded 11.2% lipid per dry biomass; hence SSOH12 cannot be classified as lipid producing strain under the chosen conditions in shake flasks with YM medium with additional glucose.
After 24 hours of incubation with tested compounds, culture medium was replaced with standard expansion medium in CTRL wells while medium with additional 2.5 mM CaCl2 in all other culture conditions.
However, on medium with additional exogenous hydrogen peroxide the PaCatB over-expressors (PaCatB_OEx1-3) disignificantnincreasedncreased growth rates.
COCs were matured in vitro in control medium and medium with additional 0.035% or 0.07% of exogenous HA.
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Regarding the strong SI, the scenario was similar to medium SI with additional exception for chromosomes 2, 4, and 20.
Dorsomorphin was added with daily changes of ES media until day 0, when EB formation was initiated in hanging drops containing EB/differentiation medium with an additional dose of dorsomorphin.
SR5 and SR40 cells were cultured in the above medium with an additional 800 μg/ml Geneticin (GIBCO) as a selective agent.
We apply the procedure to the latest Escherichia coli metabolic reconstruction, in glucose minimal medium, with an additional constraint to account for the mechanism coordinating carbon and nitrogen utilization mediated by α-ketoglutarate.
The common reactome of Francisella during infection and during growth on Chamberlain medium with glucose (Additional File S5) is comprised of genes required for growth and macromolecule formation including nucleotides and fatty acids.
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