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In sterile Erlenmeyer flasks, ca. 1.6 × 106 cells/mL from community Q63h were inoculated into minimal growth medium with added yeast extract.
2102Ep cells were grown in DMEM medium with added 100 μ M β-mercaptoethanol, and stimulated with 10 μ M RA for 0 10 days.
Batkoa sp., Furia sp. and N. floridana were similar concerning their growth patterns in a basic medium with added salts, vitamins and amino acids, as well as with a combination of all three compoments.
After three days of incubation, two of the four dishes were trypsinized and serially diluted into fresh medium, with added adenine (15 µM), for clonogenic assay of viable A549-r cells.
L15 medium with added penicillin (100 U/mL), streptomycin (100 µg/mL) (Pen/Strep).
HKCs were cultured in EpiLife medium with added human keratinocyte growth supplements (HKGS).
Similar(43)
Liquid cultures were grown in modified Sager and Granick medium I with added Hutner's trace elements (R medium) or without acetate (M medium), or in M medium lacking nitrogen (M-N medium) (Harris 1989).
When cells were recovered in selective medium (with NaCl added) alkaline-shocked, but not heat-shocked cells, also showed higher PEF resistance than control cells.
As a comparison, axenic Synechococcus sp. PCC 7002 was also maintained in BG11 medium with exogenously added vitamin B12 and 60 g l−1 NaCl.
Strains were grown in Luria-Bertani (LB) medium with antibiotics added when required.
Control cultures received fresh medium with no added ethanol or FIV.
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