The isolated HACs were then cultured in growth medium which consisted of Ham's F-12 Dulbecco's F-12 Dulbeccoe's ModifiedGibco), 10% foEaglebovine serum (FBS; Gibco), 1% of antibiotic and antimycotic (Gibco), 1% of 50 μg/ml ascorbic acid (Sigma, St. Louis, USA) in T25 culture flasks (Nunc, Denmark).
To improve the interfacial properties between carbon fibers and epoxy matrix, the fibers were treated by an ammoniac medium, which consisted of supercritical water, ethanol and triethylene tetramine.
Strain FERM P-19486 was routinely grown in the nutrient medium, which consisted of 3% (w/v) bouillon (Nissui Co., Tokyo, Japan), at 30°C for 3 d with vigorous shaking.
Calli were transferred to plant regeneration medium, which consisted of MS salt and vitamins, supplemented with 1 mg/L NAA, 4 mg/L kinetin and 3% sucrose, and gelled with 0.3% phytagel.
The highest described concentration of BS was reported by Chong-Rodríguez et al. ([2011]), who obtained an inconsistent concentration of 6.38×109 ± 3.63×109 BS/ml in a somewhat costly complex medium, which consisted of 5% sucrose, 2% corn steep liquor and basal salts.
ES cells were cultured on dishes coated with 0.1% gelatin (Sigma) without feeders in 60% conditional Buffalo Rat Liver (BRL) medium, which consisted of 60% BRL medium and 40% standard ES medium.
Artificial wastewater is actually a synthetic medium, which consists of those inorganic nutrients present in real wastewaters, but lacks toxic chemicals and microbes, and characteristics of real wastewaters.
We consider a linearly elastic composite medium, which consists of a homogeneous matrix containing a statistically inhomogeneous random set of heterogeneities of arbitrary shape.
One considers a linear thermoelastic composite medium, which consists of a homogeneous matrix containing a statistically homogeneous random set of ellipsoidal uncoated or coated heterogeneities.
Rev., (2001a), 54, 1–47) based on the theory of functions of random variables and Green's functions is used for demonstration of the dependence of effective elastic moduli of fiber composites on the radial distribution functions estimated from the real experimental data as Well as from the ensembles generated by the method proposed.
Nanocomposite is modeled as a linearly elastic composite medium, which consists of a homogeneous matrix containing a statistically homogeneous random field of homogeneous prolate spheroidal nanofibers with prescribed random orientation.
