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Second, hBMCs but not hPDCs displayed spontaneous ALP activity in growth medium when seeded at high density.
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We did not detect any significant difference between Dox-treated and untreated HuH6sh CTNNB1 when seeded at medium density (Additional file 2: Figure S1-D).
Cells were more optimally distributed throughout the conduit when seeded in fibrin than by delivery in growth medium alone.
Caco-2 cells showed an enhanced ability for clonal growth when seeded on a type I collagen-coated surface in serum-free medium.
Brain endothelial cells form highly branched networks when seeded on matrigel and this was unaffected when GST was added to the culture medium.
Consistent with this, more MuSCs were obtained after 48 h of in vitro culturing in conditional medium when the same amounts of cells were seeded.
To investigate this scattering phenomenon further, we seeded cells at low density and changed medium when the cells were approximately 25% confluent.
Cells were initially seeded at a density of 1 × 10 cells/cm in normal medium, then converted into specific differentiation medium when they approached 80-90% confluence.
However, when U251 cells cultured in serum-free medium were seeded in serum-containing medium for 48 h, almost all expressed GFAP while nearly none expressed nestin.
In contrast, parts of the transgenic seeds of OsRFP-EGFPs germinated faster than control seeds when seeds were plated on 1/2 MS medium supplemented with NaCl (150 mM) and PEG (−0.7 MPa; Figs 7 and 8).
Cancer stem cells are morphologically identified because of their ability to grow as 3D nonadherent structures when clonally seeded in sphere medium.
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