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The presence of an 80 kDa CGB-immunoreactive band was detectable among proteins in the extracellular medium when cells were treated with nicotine.
Schönheit et al. discovered that at low concentrations of nickel (less than 100 nM), cobalt (less than 10 nM), and molybdenum (less than 10 nM), the quantity of Methanothermobacter marburgensis cells formed was roughly proportional to the amount of transition metal added to the medium when cells were grown on H2 and CO2 [46].
Serum was withdraw from the culture medium when cells were 70% confluent.
Samples were harvested 5 hr post transfer to sporulation medium, when cells were arrested in the NDT80 block.
For the treatment, MSeA at a concentration of 5 μM as added into the DMEM medium when cells reached 60-70% confluence.
Sgo1-3V5 localization was determined by ChIP-chip, 7 hr after transfer into sporulation medium when cells were arrested in metaphase I. Arm peaks for Sgo1 correspond to cohesin-associated regions.
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Intracellular enzyme lactate dehydrogenase is released into the cell culture medium when cell membrane damage occurs.
DMSO, ATTM, and 2ME2 were supplemented into the medium when cell indices attained ∼20 25% of their untreated maximum values (∼24 hr postseeding) and growth was monitored for 3 additional days.
To investigate this scattering phenomenon further, we seeded cells at low density and changed medium when the cells were approximately 25% confluent.
To identify the essential role of CA1 for calcification in breast cancer cells, acetazolamide, a chemical inhibitor of CA1, was added into the culture medium when 4T1 cells were induced to produce calcification with OC.
LDH is rapidly released into the cell culture medium when the cell plasma membrane is damaged.
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