Sentence examples for medium were washed from inspiring English sources

To assess differentiation, cells cultured in differentiation medium were washed with phosphate-buffered saline (PBS) and fixed in 4% paraformaldehyde.

Two-day-old mycelia, cultured in the nutrient pea broth liquid medium, were washed in 0.8 M mannitol, then placed in enzyme solution, and incubated for 40 min at room temperature with gentle shaking.

Alexa488-OVA beads were added to cells at 20 1 in complete medium, were washed in modified Ringer's after 30 min, and imaged using alternate 555/590, 488/530, 488/590 nm Ex/Em and brightfield illumination 30 and/or 90 min after addition.

OxyBurst/Alexa568-OVA zymosan were added to cells at 20 1 in complete medium, were washed in modified Ringer's after 30 min to remove uningested zymosan, and imaged using alternate 555/590, 488/530 nm Ex/Em and brightfield illumination 30 and/or 90 min after addition.

For serum activation, cells in low-serum medium were washed twice with PBS, and DMEM medium containing 10% FBS was added to the cells and incubated for 4 hours.

C. neoformans cells grown in YPAD medium were washed three times with PBS then resuspended to a density of 5×106 cells/ml in PBS, and 10 µl (5×104 cells) were co-incubated with the RAW264.7 macrophages in 200 µl fresh DMEM.

The growing medium was washed off from the roots and dry weight of the roots was taken as below-ground biomass.

A wound was created by scratching cells in 1.5-cm zone with sterile 200-μl pipette tip after the old medium was washed out.

While the nanoparticle/DNA in the medium was washed away at 24 h after transfection, after another 24 h, GFP signals were not obviously enhanced (data not shown).

36 hours post transfection, medium was washed off and cells were mixed.

After 1 h incubation, the medium was washed well and cultured for a further 48 h.