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(B) Cell lysates under full (SD) medium were treated with lambda PPase at 30°C for 0 h, 0.5 h and 1 h, then samples were assessed by Western blotting with HA antibody.
For co-culture fermentation (F3 and F4), separate sets of reaction mixtures consisting of pretreated WHB supplemented with basal medium were treated with P. stipitis simultaneously with T. reesei (F3) and F. oxysporum (F4).
Samples in LB medium were treated for 15 min with a total current of 0, 1500 500, or 1500 μA (corresponding to 0, 25, 83 and 250 μA/cm2, respectively) in the electrochemical cell described above.
After overnight cultivation, only flasks with a clear medium were treated as follows.
10-day-old Arabidopsis seedlings grown on MS medium were treated, or not treated, with NaCl solution (200 mM).
M. bovis BCG cultures grown in Sauton's medium were treated with 1 µg/ml of either TAC or SRI-224 for 5 days.
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Rarely has the medium been treated with such brio, such abandon and such up-front sass.
But as Twitter-defenders have pointed out, following CPS action on racist remarks and terrorism gags, this policeable medium is treated carelessly by millions of easily identified users as a safe, semi-private repository for uncensored stream of consciousness.
First, the case of a purely anelastic (Kelvin Voigt) heterogeneous medium is treated.
In this model, the MEE medium is treated as being mechanically brittle, and electrically and magnetically ductile.
It's about how the medium is treated, such as in the press, and how it is in the ludic century that companies like Apple still treat games as second class culture.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com