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When the 30Kc6-expressing cells cultivated in the serum-containing medium were transferred directly to commercially available serum-free media, 30Kc6 expression increased the viable cell density by four-fold through inhibiting serum deprivation-induced apoptosis.
The flasks containing the cells in fresh medium were transferred aseptically to the bioreactor to start new batch fermentation.
(b) Radicle emergence was recorded and data are means ± SD (n = 30 35 plants) (c) One-week-old seedlings grown on standard medium were transferred to culture plates with concentrations of mannitol (0 300 mM) for 7 days.
Seven-day-old seedlings grown on MS medium were transferred to the medium with various NO3 −, Ca2+ and K+ concentrations, and grown for 14 days in a growth chamber at 24 °C under 8 h of darkness and 16 h of light at 150 μmol photons m−2 s−1.
Yeast transformants (Leu+, Trp+, His+) grown on minimal medium were transferred onto filter papers.
7-day-old seedlings grown on nutrient solid MS medium were transferred to MS liquid medium plus or minus 20 mM imidazole for 4 h.
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After the incubation, the reaction medium was transferred to ice and centrifuged at 10,000 rpm for 30 min at 4 °C.
Before the reagent was added, 3.0 mL of cultured medium was transferred to other dishes at 37 °C, and the membrane was transferred to each medium in turn (1 mL reagent medium for 30 min; 1 mL wash medium for 10 min; and 1 mL remaining medium for monitoring of bioluminescence).
Conditioned medium was transferred to naïve cells, and viral p24 protein in the culture supernatant was measured by ELISA.
The mycelium stored on PDA medium was transferred to new PDA medium in 9-cm diameter Petri dish and incubated at 30°C for 5 days.
After 70 h, each medium was transferred to a 500 mL screw-cap flask and centrifuged at 3000 rpm for 20 minutes.
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