Sentence examples for medium were transfected from inspiring English sources

Ten million cells with 400 µl medium were transfected by electroporation with a Bio-Rad Gene Pulser in 0.4 cm-gap cuvettes at 210 Volts and 975 microfarads.

HEK 293 cells starved for 24 h in steroid-free medium were transfected using Lipofectamin 2000 (InVitrogen) with pcDNA3-hGRα or pcDNA3 hGRα-R469X together with pSVbgal and pGRE2-TATA-luc plasmids and then exposed to 100 nM DEX.

Arabidopsis mesophyll protoplasts were prepared as described above and 104 protoplasts in 100 µl growth medium were transfected with 10 µg pGFP2-C4 vector in which C4 also fused with GFP at the N-terminus under the control of 35S promoter.

In the parallel approach HEK cells, grown in normal medium, were transfected with STAT3 without the tag for biotinylation.

U251 cells cultivated in the control medium were transfected with pmaxGFP plasmid using Nucleofection™ technology (Lonza Group Ltd., Switzerland).

C2C12 cells cultured in the growth medium were transfected individually with miR-133a mimics, miR-133b mimics, Duplex NC or both miR-133a mimics and miR-133b mimics.

Approximately 106 A549 cells cultured in complete Dulbecco's modified Eagle's medium (DMEM) were transfected with 2 μg pcDNA3.1 with FuGene HD, according to manufacturer's protocols.

After overnight culturing in serum-free medium, cells were transfected with scrambled vector (SV), puPAR (pU), pCathepsin B (pC), or a bicistronic construct comprised of puPAR and pCathepsin B (pCU) for 72 hrs using FuGene according to manufacturer's instructions (Roche Applied Science, IN).

CHO-K1 cells (DSMZ), adapted to grow in serum-free UltraCHO medium (Lonza), were transfected with the S1PR3-Gα15 S1PR3-Gα15d stably transfected cells were selected by treatment with blasticidin.

Dissociated cultures of SCG neurons from newborn C57/CD1 mice were grown in polyornithine/laminin coated 35 mm tissue culture dishes in defined medium and were transfected using Invitrogen Neon microporation kit prior to plating (Gutierrez et al., 2008).

293T cells grown in 10 % FBS Dulbecco's modified Eagle's medium (Gibco) were transfected with E2F1 or scrambled control shRNA lentiviral vectors and MISSION lentiviral packaging mix (Sigma) using Fugene 6 in serum-free OPTI-MEM medium (Invitrogen, Carlsbad, CA, USA).