Sentence examples for medium were switched from inspiring English sources

TS cells grown to confluence in TSMFH medium were switched to TS medium to induce differentiation.

Among cells that were grown in Mel-mix medium without PMA, c-Kit expression appeared in fewer cells, and when c-Kit positive cells from PMA containing medium were switched into Mel-mix, the expression of c-Kit protein in the cells decreased dramatically.

For preparation of protein extract from yeast cells, EGY48 cells transformed with the isolated pJG4-5/HeLa clonecodingcoding the FAF1 sequence obtained from 5 ml overnight cultures grown in Glu/CM-W medium were switched into Gal-Raff/CM-W for at least 8 h with vigorous shaking for induction of HA-FAF1 protein fusion under the GAL1 promoter.

24 h later, the medium was switched to CDF12 medium with medium changes every day.

When the medium was switched to adipogenic and osteogenic differentiation medium, the MSC concentrations were approximately 30, 60, 80, and 100%, respectively.

After 48 h, the medium was switched to α-MEM with 2% (v/v) fetal bovine serum (FBS) in order to initiate differentiation.

Then the medium was switched to the RPMI1640 medium supplemented with Albumin, Ascorbic acid, transferrin, selenite, 5 ng/mL BMP4 (R&D Systems), and CHIR99021 to induce cardiac mesoderm formation.

Once a stable current was observed, the synthetic medium was switched to the desired PDWW concentration buffered with 50 mM KH2PO4, pH 7.0 (Jiang et al. 2009), and MFCs were operated in continuous mode for 60 days.

Upon reaching about 90% of cell confluence, the culture medium was switched to differentiation medium consisting of DMEM supplemented with 1% penicillin/streptomycin and 2% horse serum.

Another 24 h later, the medium was switched to ESC medium [31] for a total period of 10 d, with replacement on alternate days.

Then the medium was switched to 2 ml of medium containing 100 nM of α-amanitin and time –lapse movie acquisition was continued.