Sentence examples for medium were suspended from inspiring English sources

Briefly, clinical isolates growing on Ogawa medium were suspended in 1 ml of sterile distilled water, and the suspension subjected to the test.

Cells from the agar medium were suspended in enrichment broth (MHB) to an optical density at 600 nm of approximately 0.9 1.0.

Fourteen day old, fresh M. tuberculosis cultures grown on L-J medium were suspended in 7H9 broth medium to achieve a turbidity of McFarland No.1 standard.

Briefly, 2 × 104 cells in 20 μl of culture medium were suspended on the lid of tissue culture dishes containing 10 ml of culture medium for 48 h to form spheroids.

For embryoid body formation, 10 ES cells/0.03 ml LIF-free culture medium were suspended in hanging drops and maintained for 2 3 days, and/or transferred to uncoated Petri dishes and cultured in suspension in the same medium.

Frozen E. coli cell pellets (∼0.4 g wet weight from 200 mL of medium) were suspended in 3 mL of extraction buffer (50 mM N-[tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid (TES), pH 7.0, 10 mM MgCl2, 20 mM DTT) and lysed by sonication.

To test the fraction of diploid cells from a colony on selection medium, colonies were suspended in water, plated on rich medium, and 100 single colonies were transferred to selective media.

Assays for SOD and GPx activities After removing the medium, Leydig cells were suspended in 10 mM PBS (pH 7.0-7.2, 100-200 μL/10 cells) and cells lysed via homogenization.

For Western blot analysis, yeast cells harboring carboxy-terminal epitope-tagged proteins carrying either myc or HA tags (the proteins Cdc5, Aco1, and β-actin did not carry a tag and were detected with appropriate direct antibodies) were grown for 24 hr in liquid YPD-rich medium and, upon saturation, were suspended in sporulation medium (SPM) at a titer of 2 × 10 cells/ml and shaken vigorously.

After trypsinization, cells were suspended in medium 199, and the culture medium was returned.

After 1 week of induction the culture was centrifuged, the conditioned medium was harvested and the cells were suspended in fresh induction medium to start another round of induction.