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Ten days later (when plants were seven days old), the medium were supplied with 5 μM H2O2, 5 μU/ml catalase, or both and with sterile water in the control group.
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The sugar-containing medium was supplied in an upstream mixing chamber, where the pH value was adjusted to 7.2.
For a mammalian cell culture exposure at the ALI, the medium is supplied individually for each of the 4 well compartments and does not transit between them.
The special technological medium is supplied into the friction zone to play two roles: (a) alloying the surface layer with chemical elements present in the medium due to its thermal destruction within the contact zone and intensive mass transfer and (b) the structural-phase transformations in the material during its rapid cooling.
Other systems are restricted to very early developmental stages such as the rolled paper systems where the medium is supplied by capillarity in the support paper and that give access to a bi-dimensional view of the early root system still anchored on the paper after opening (Woll et al. [2005]).
For cultivation of simian cells, culture medium was supplied with 10% autologous serum.
New medium was supplied every 3 4 d and the adherent cells (>95% CD11b+F4/8 0+ harvested after 7 14 d.
YOYO-1, a hydrophobic bis-intercalator quenched by aqueous medium, was supplied by Molecular Probes (Eugene, Oregon, USA).
Williams' E medium was supplied by Eurobio (Les Ulis, France) and fetal calf serum (FCS) by Perbio (Brebieres, France).
Cells were washed and fresh medium was supplied.
Fresh medium was supplied three times per week.
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