Sentence examples for medium were substituted from inspiring English sources

In order to initiate the maturation phase, the cytokinins and auxin in the liquid GD medium were substituted with 20 μM abscisic acid (ABA, Sigma-Aldrich) and 3.75% polyethylene glycol 4000 (PEG, Sigma-Aldrich).

When another medium is substituted, as in boards painted to look like stone, the result looks fake.

When indicated, the 2% glucose of the defined medium was substituted by glycerol (in fact, 2% glycerol and 0.2% glucose).

At these time points, culture medium was substituted by RHB-A: Neurobasal: B27 (1∶1∶0.02) medium supplemented either with 0.01% DMSO (control) or with 3 nM LY411575 (in 0.01% DMSO).

On day 2 (d2), the serum containing medium was substituted with serum replacement medium (KO) and this resulted in initial proliferation and a gradual switching to differentiation.

The next day, the medium was substituted with DMEM/F12 containing 0.1% FBS with or without siRNA to render the cells quiescent.

The medium was substituted with Knockout-DMEM (Kon on d2 that contained all the additives and Knockout serum replacement (Invitrogen) in place of FBS and was supplemented with (KR) or without 100 nM all-trans retinoic acid (RA).

After 1 h incubation, the medium was substituted with ordinary growth medium.

Culture medium was substituted twice a week and cells were harvested after reaching 70 80% confluence.

After 15 min, the incubation medium was substituted for the same solution containing collagenase only.

The medium was substituted by fresh DMEM, 10% FBS (3 ml).