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Extracellular vesicles from urine and cell culture medium were subjected to differential centrifugation as previously described22.
Aliquots of the culture medium were subjected to ELISA analysis of IL-6, IL-10, and IL-12 levels.
To quantify the inflammatory cytokines (TNF-α, IL-2, IL-6, IL-10, IL-12p70, MCP-1, IFN-γ), 50 µl of culture medium were subjected to CBA kits by using FACS analysis according to the manufacturer's instruction.
In order to assess the effect of nicotine stimulation on SH-SY5Y cells, morphine concentrations in the medium were subjected to post hoc analysis using a Mann-Whitney test.
Lysates and medium were subjected to immunoblotting analyses.
Samples of conditioned medium were subjected to enzyme-linked immunosorbent assay for determination of IFN-γ.
Similar(43)
It is assumed that the medium is subjected to a harmonic surface load of given frequency.
The top surface of the layered medium is subjected to dynamic in-plane surface tractions; whereas, the lower surface is free or fixed.
The medium is subjected to stress free, thermally insulated, electrically shorted/charge free boundary conditions and is rotating about an axis perpendicular to its plane.
Dynamic stresses around two equal collinear cracks in an infinite elastic medium are evaluated based on linearized couple-stress theory while the medium is subjected to time-harmonic stress waves impinging normal to the cracks.
To verify the proteomic results, conditioned medium was subjected to sequential centrifugations, ending in a 120,000 g ultracentrifugation.
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