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Aliquots of medium were stored at –80°C until analysis.
Whole stool and inoculated transport medium were stored at 2 8°C for up to 3 days before transport to LNSP.
The conditioned medium was collected after 72 h of incubation and centrifuged at 2000 g for 10 min, and aliquots of cultured medium were stored at −80 °C before analysis.
Peripheral blood mononuclear cells (PBMCs) were isolated from on-study blood collections by centrifugation over lymphoprep™ (Axis-Shield PoC AS) as described above; 5 10 × 10 viable cells/mL freezing medium were stored in liquid nitrogen.
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The medium was stored at 4 °C without delay.
The medium was replaced on day 1 and 3; and the retrieved medium was stored at −20 °C until progesterone assay.
The supernatant was removed and the wells were washed with DMEM-F12 without inactivated FBS; this conditioned medium was stored to be used later.
This concentrated medium was stored at −20°C in aliquots.
The transport medium was stored at 4°C until use.
The conditioned medium was stored at -20°C until analysis.
After vortexing, the swab was discarded and the medium was stored at -80 °C.
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