Sentence examples for medium were stimulated from inspiring English sources
Purified DCs (1 × 104 in 200 μL culture medium) were stimulated with different TLR ligands at the concentration mentioned above.
DCs were enriched by lineage (CD3, CD14, CD16, CD19) depletion, then lineage negative cells (2 × 105 in 200 μL culture medium) were stimulated with TLR ligands or viruses for 4 h followed by another 2 h in the presence of Golgi Blocker (BD Biosciences, Franklin Lakes, NJ, USA).
Differentiated T37i cells, starved for 18 h in minimum medium, were stimulated with 100 ng/ml PRL, during 0, 5 and 15 min; cells were washed twice with ice-cold PBS and lysed as previously described [18].
PBMC (6×106 cells/ml) in culture medium were stimulated for 18 to 20 hours with peptides (PfCS102, 10 µg/ml, plus HLA-A2 and/or HLA-A3 CTL peptides, 5 µg/ml each) in the presence of brefeldin A (10 µg/ml, Sigma) during the last four hours of culture.
D407 cells, starved for 24 hours in serum-free medium, were stimulated with 100 nM Tat for different time durations.
Jurkat E6.1 cells (2 × 10 per ml RPMI 1640 medium) were stimulated with gal-1 (20 μg/ml) at 37°C and washed once with ice-cold phosphate-buffered saline (PBS).
Their hybrid medium was stimulated by an advance in photography: the invention of the carte-de-visite process, which was patented by the French photographer André Disdéri in 1854.
The growth of Oncidesa (formerly Oncidesa Gower Ramsey 'U-1') plantlets on Vacin and Went (VW) medium was stimulated by 10 000 μmol mol−1 CO2.
CD14+ monocytes were resuspended in RPMI 1640 medium supplemented with 10% fetal calf serum, 2mM glutamine, 50 U/ml penicillin, 50 μg/ml streptomycin (complete medium) and were stimulated with varied concentrations (0.001μM to 10 μM) of benzo(a pyrene (B aP) or equivalent dimethyl sulfoxide (DMSO) alone (control) for 24 hr.
The MM cells were kept in serum-free medium and were stimulated with IGF-1 for 10 min.
After serum starvation for 16 in the absence of L292-conditioned medium, macrophages were stimulated with human M-CSF (100 ng/ml) for 1 14 h.
