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Without hESC extract treatment, epigenetically modified HFFs in mTeSR™1 medium were spread out and exhibited characteristic fibroblast morphology, whereas cells treated with the inhibitors and hESC extracts appeared to be short and flattened, without cell-cell contact (Figure 4A (a) and (b)).
Aliquots of 0.01 ml of each sample (in the thioglycollate medium) were spread on Columbia blood agar plates containing 5% bovine blood.
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Assay plates were similar to those used for exploratory locomotion except 50 µL of OP50 E. coli cultured in Lysogeny Broth (LB -Miller medium was spread onto the pLB -Milleray before use.
Then, to select the transformants, cultured bacterial LB mediums were spread on 1.5% LB agar dish containing ampicillin (100 µg/ml) and incubated overnight.
Library diluted by LB medium was spreaded onto LB/Cm plates which were inverted and incubated at 37°C overnight.
After addition of 1 ml of cold LB medium, bacteria were spread onto LB plate containing 100 μg/ml ampicillin and incubated at 37°C.
After recovery for 1 h at 37 °C in 600 μL of SOC medium, cells were spread on LB agar plates containing 200 μg/mL ampicillin and incubated overnight at 37 °C.
Xylene, octane, and naphthalene were added in the inner side of the lids of Petri dishes and incubated upside down to allow the upwards diffusion of the HC through the medium, whereas the other HCs were spread on the medium surface.
After the incubation of the yeast mixture in the medium containing newspaper, yeasts were spread on a SD+M plate.
After incubating for 5 days under antibiotic treatment, serial dilutions were made in Middlebrook 7H9 liquid medium and appropriate dilutions were spread on Middlebrrok 7H10 agar plates without any antibiotic.
For soil plates, 2 g of soil was placed in a sterile Petri dish, cooled PDA medium (15 mL) was added, and soil particles were spread in the medium.
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