Non-supplemented DW medium was spiked with several N sources (Table 2): no supplement as a reference, (NH4 2SO4 as the usual N supplement, (NH4 2CO3 as an alternative ammonium resource, and urea as another N compound.
When medium was spiked with Cd, with an increase in Cd concentration, there was an increase in GST activity in both roots and shoots of transgenic tobacco plants up to 100 µM Cd (Figure 4A, B).
Usually, the medium was spiked with benzene (120 μM) or benzoate (700 μM) as carbon and energy sources, respectively.
Briefly, 20 μL of a 5 mg/mL solution of MTT in serum-free medium was spiked into each well, and the plates were incubated for 4 h.
Standard aqueous quality control stock of LND each at low, medium, and high concentration levels were spiked in plasma and millipore water separately, the later being considered as unextracted QC samples.
For NIR measurements of the heat-shocked cells, heated bacteria were spiked into fresh medium and immediately transferred into one test chamber of the cassette; the adjacent control chamber was filled with the same medium spiked with PBS.
To investigate the traceability under cell culture conditions, stressed antibody samples were spiked into SFM4CHO medium or CHO DG44 host cell supernatant and analyzed immediately afterwards.
Three of those samples were spiked on one medium concentration level by adding an aliquot of the multi-analyte stock solution prior to extraction.
The multiple libraries of BLM-1 and BLM-2 mixtures were spiked with either of these pools at one of three (high, low, medium) concentrations.
Samples were spiked with Agilent One-Color Spike-Mix (1 10).
