Sentence examples for medium were solubilized from inspiring English sources

The fluorescent proteins in 10 ml E. coli grown in LB medium were solubilized by BugBaster (Novagen) and purified by Talon column (Clontech).

Assay was stopped by removing the medium on ice and membranes were solubilized using KIRA-ELISA solubilization buffer (NaCl 150 mM, Hepes 50 mM, Triton X-100 0.5%, thimerosal 0.01%, sodium orthovanadate 2 mM, supplemented with a cocktail of protease inhibitors) for 1 h at room temperature.

Removed medium and formazan crystals were solubilized by adding 150 μL DMSO.

After aspirating the culture medium, the formazan crystals formed were solubilized with 200 μL of organic solvent.

Test samples were solubilized in medium to the desired treatment concentration and cells were treated with 100 μL of each solution.

Cell culture in medium was used as a negative control, R, and quercetin 50 µM as a positive control, R. Extracts and quercetin were solubilized in culture medium.

After the addition of 20 μL MTT solution (5 mg/mL) per well, the plates were incubated for 4 h, the medium were removed, the formazan crystals were solubilized in 100 μL DMSO per well and the absorbance values were read at 570 nm.

Thereafter, the culture medium was removed and the cells were solubilized in 100 mL DMSO.

Then the medium was removed and formazan crystals were solubilized with 200 μl of DMSO and the absorbance was evaluated by a microplate reader (BIO-RAD MODEL 680, USA) at 492 nm.

The medium was then removed and the cells were solubilized in isopropanol.

The medium was removed, and the MTT crystals were solubilized with 50% DMF.