5 × 103 3T3-L1 preadipocytes suspended in 100 μL of growth medium were seeded in 96-well plates and let cells grow to confluence.
3T3-L1 preadinocytes in 100 μL growth medium were seeded in 96-well plates at a density of 5000 cells per well.
About 4 × 107 osteoblast precursor cells in 200 μl cell-culture medium were seeded into each of six scaffolds and incubated in vitro for 2 days.
For epifluorescence microscopy experiments, 2,000 cells/well in 500 μL/well of culture medium were seeded onto 11-mm glass coverslips in 24-well culture plates with 10.0 μL of CNT/DNA conjugate solution added to the wells.
Briefly, 2×104 HUVEC resuspended with 500 µl of EBM-2 medium were seeded on Matrixgel solidified in 48-wel tissue culture plate.
Bacteria cultured in BH medium were seeded for 24 hours in DMEM (Gibco) containing 10% fetal calf serum (FCS) and 2% inulin.
Briefly, 2×104 HUVEC resuspended with 500 µl of EBM-2 medium were seeded on Matrixgel solidified in 48-well tissue culture plate.
HT1080 cells (5×103) in 100 µl of FBS-free medium were seeded into the upper chamber and incubated at 37°C in a 5% CO2 incubator for 6 h.
MV3 or Lifeact transfected MV3 cells in 2 ml medium were seeded at a density of 4×104 cells/ml and left to spread overnight at 37°C and 5% CO2 in a humidified atmosphere.
1×105/well HBMEC in 200 µL medium were seeded onto polycarbonate membrane inserts (pore size 0.4 µm, surface area 0.33 cm2) purchased from Corning Inc. (Cat. no. 3413, Corning, NY).
Mixl1 GFP ES cell-derived EPL cells maintained in aggregates and cultured in medium supplemented with MEDII, 50% E or unsupplemented medium were seeded on day 7 and allowed to differentiate (Figure 4B).
