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S. pombe cells growing exponentially in YE medium were sampled and total RNA was prepared from each sample.
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At specific times, the medium was sampled for analysis of drug concentration.
The culture medium was sampled every 7 days to monitor pH and metal elution from SMS.
At predetermined time intervals, the release medium was sampled and replaced by an equal volume of fresh buffer to maintain a constant volume.
To determine the secretion of IL-2, 150 µL of the medium was sampled at 3, 5, and 7 days of incubation and frozen at −70 °C for subsequent measurement.
To determine the release of IL-2 from lymphocytes against the islets, 100 μL of the medium was sampled on the fifth day of culturing and frozen at −70°C for subsequent measurement.
In order to assess the release of Zn within the first half of the 7-day exposure, the cell medium was sampled after 3 days of cell exposure, then the cell medium was replaced with a fresh one and again sampled after another 4 days (at the end of the exposure).
The incubation medium was sampled and radioactivity was counted.
Test medium was added and left for 20 h, and medium was sampled and counted.
At predetermined time intervals, 1 mL of the medium was sampled for further analysis by HPLC.
The release medium was sampled (7 mL) at predetermined time intervals and analyzed by UV spectroscopy at 630 nm.
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CEO of Professional Science Editing for Scientists @ prosciediting.com