Sentence examples for medium were resuspended from inspiring English sources

P6 pellets derived from C15 and C17 cell conditioned medium were resuspended and incubated with either anti-HLA class II coated beads or control beads.

To measure cellular autofluorescence by using flow cytometry, cells growing in shaking culture in HL5 medium were resuspended in PBM at 1×10 cells/ml, and were incubated for 16 hours at 20°C on a rotary shaker.

Pelleted cells from 20 L medium were resuspended in potassium phosphate buffer (600 mL, 100 m m, pH 6.2), and substrate 3-methylfuran-2 5  H)-one (1 a; 2 g, 20.3-methylfuran-2 5d. 3-methylfuran-2 5

Salmonella grown overnight in LB medium were resuspended in 0.15 M NaCl and administered to mice via the i.p. route of infection (dose 10 or 10 CFU) (Heithoff et al., 1999).

Yeast colonies grown onto -LW medium were resuspended into Z buffer (Na2HPO4, NaH2PO4, KCl, MgSO4) at an OD600 between 0.5 and 0.7 and concentrated in a final volume of 300 μL.

To obtain genomic DNA for spoligotyping and MIRU-VNTR typing, mycobacterial colonies grown on LJ medium were resuspended in 100uL 1X Tris-EDTA buffer (10 mM Tris-HCl, 1 mM Ethylenediaminetetracetic acid disodium [pH8.0]) and then boiled for 30 minutes.

In the first experimental setting, lyophilized rMNC apo sec (produced from 12.5×10 apoptotic rat MNCs) or control medium was resuspended in 0.3 mL saline (Fresenius Kabi, Vienna, Austria) in the laboratory prior to surgery.

After several washings in RPMI 1640 medium, cells were resuspended in complete medium (RPMI 1640 supplemented with 10% FBS, l00 U/ml penicillin G sodium, 100 mg/ml streptomycin sulfate and 50 mM β-mercaptoethanol [Sigma-Aldrich]).

The cells were then washed three times in DMEM F12 medium and were resuspended in mammosphere culture medium comprising DMEM F12 with the serum replacement supplement B27 (Invitrogen, Paisley, UK) and hydrocortisone, insulin, epidermal growth factor and bovine pituitary extract (SingleQuots; Cambrex Bio Science, Nottingham, UK).

Following centrifugation to remove medium, cells were resuspended in 0.2× phosphate buffered saline, boiled for 10 min, and mixed with 0.1 volume of 10-mg/ml proteinase K (Sigma, Marlborough, MA), and the mixture was incubated for 30 min at 55°C.

After removal of the medium, crystals were resuspended in 100  μl DMSO.