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Exact(14)
Twelve hours after infection, the cell medium were replaced with fresh complete medium, and puromycin was added 72 h post infection to select stable cell lines.
After these pre-incubations, different concentrations (1 µM-100 µM) of calycosin medium were replaced.
Five hours later, the culture medium were replaced with either Zn-free medium or medium supplemented to 65 M ZnCl2 to induce asymmetric self-renewal.
Cultures were treated at DIV1 and every four days 300 µL of culture medium were replaced by medium supplemented with treatments.
Subsequently, the culture medium were replaced with fresh medium.
After 4 6 h, the medium were replaced with 10% FBS DMEM.
Similar(46)
The medium was replaced twice a week.
After 24 h, the medium was replaced.
The medium was replaced 12 h later.
The medium was replaced every second day.
Mature Medium was replaced every 2 days.
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