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Many works using this medium were produced but few survive today because of the delicateness of the linen cloth and the solubility of the hide glue from which the binder was derived.
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In air fluidization technique, autogeneous medium is produced by mixture of air and fine coal power which behaves as a separating density.
WEHI-3B-conditioned medium was produced by seeding WEHI-3B cells (0.5×106 cells/mL) into the medium described above and incubating them for 3 days.
Conditioned medium was produced in transiently transfected HEK293T cells, and TOPFLASH/FOPFLASH (Upstate, Lake Placid, USA), pTOPGlow/pFOPGlow [34] TCF luciferase reporter genes or DKK1 promoter luciferase constructs WT short and TBE3,4mut short [38] were transfected to MCF7 cells as described for sHPT-1 cells [26].
AFP and ALB in the extracellular medium is produced and released into extracellular fluids by cells.
The treated LB medium was produced by growing the pBSK-P16S-g10-mt1-rpsT bacteria clone in LB medium containing 120 μM Hg for 120 hours.
Wnt3a conditioned medium was produced from mouse L-cells (ATCC) stably expressing the recombinant protein and used to treat HeLa cells.
Wnt3a conditioned medium was produced using stably transfected L cells after 1 week of conditioning in medium (as previously described Sato et al, 2009) containing 10% fetal bovine serum.
Spent medium was produced by growing T. saccharolyticum ALK2 on 5 g/L cellobiose MTC medium in an anaerobic glove bag (Coy Laboratory Products, Grass Lake, MI) in the presence of 62.5 g/L Avicel.
In addition, we show that kynurenine in the coculture-conditioned medium is produced mainly by CAFs because IDO is not induced by COX-2 overexpression in MCF-7 cells.
IDO was very low or undetectable in MCF-7 and COX-2-overexpressing MCF-7 cells, indicating that the kynurenine in the co-cultured medium was produced mainly by RMF-EG cells.
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