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Two feedstock powders with different particle size distributions, dispersed in isopropanol medium, were processed by setting up the HVSFS torch with combustion chambers of different lengths and with two distinct injection systems: a water-cooled mechanical injector, delivering a continuous stream of suspension, or a gas-atomiser injector.
(D) Protein extracts of dark-grown seedlings from 0.5%% sucrose-supplemented medium were processed for immunoblotting.
The cell lysate and medium were processed to assess secretion of Collagen VII as described for RDEB/FB/C7 cells.
The raw file images obtained from plants grown on GM medium were processed using Adobe Photoshop CS3 (San Jose, CA, USA).
Cells were initially treated with DRB for 3.5 hr as described above, and samples from time points 10, 25, and 40 min after release into fresh medium were processed.
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Uninoculated culture medium was processed as a negative control.
The crossed purification on the solid medium was processed until the strains with the same morphology were examined by microscope.
After incubation the medium was processed for the quantification of 11-KT [34].
Extracellular medium was processed as described before and was used for immunoprecipitation and for purification of exosomes.
Conditioned medium was processed by copper binding followed by gel filtration.
Blanks (wells containing medium alone) were processed in parallel, and their values were subtracted from those of the sample plates.
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