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> -wrap-foot> Well-developed rooted plantlets formed either from direct shoot production from axillary buds or indirectly from PLBs in the induction medium were planted out in pots containing one of six compost recipes and grown on in a glasshouse for 90 days.
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Each jar (containing about 700 mL of soil mixture) was watered with 125 mL quarter-strength nitrogen-free Jensen medium [ 33] before seeds were planted, three plants per jar.
In each line wetlands had a surface area of 2.80 m2, a water depth of 25 cm and a granular medium D60 = 7.3 mm, and were planted with common reed.
Pak-Coi mustard seeds were planted in the medium mixture of cow dung and soil with a ratio of 1 1.
One column of each aggregate or medium type was planted.
Butterfly was planted in a medium that contained 25 mg/kg cadmium (Cd) with application of varying nitrogen concentrations (0 mM, 8 mM, 16 mM, 24 mM and 32 mM) weekly.
Developing shoots were subsequently transferred to a root initiation medium CR5 [ 36] before finally being planted into potting mix in the greenhouse.
Each wetland had a surface area of 2.95 m2, a water depth of 25 cm and a granular medium of D60 = 7.3 mm, and was planted with Phragmites australis.
Each experimental plot was planted five lettuces with rockwool as planting medium.
The source of organic carbon in our liquid prey culture medium was filtered plant detritus (hay extract, Ward's natural science, Rochester, NY), for a detailed recipe and methods for culture media preparation see [ 17, 18].
Induction in liquid medium was chosen because wildtype plants metabolised proline very quickly when it was provided via the solid culture medium and only marginal increases in proline content were observed in the leaves.
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