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The internal parchment membrane of the air chamber was dissected, and a small silicone ring was placed on the blood vessel area, and 5 × 106 of tumor cells suspended in 20 μl drop of medium were placed into the silicone ring.
Briefly, cells (1.5×105 cells/well) suspended in serum-free medium were placed in the upper chamber of the Transwell insert, and RPMI 1640 medium containing 10% FBS was added to the lower chamber.
One hundred and fifty µL of different medium was placed in the lower chamber of the trans-well system and 5×104 HUVEC in control medium were placed above the filter and incubated for 12 hours at 37°C.
Four drops of 100 μL maturation medium were placed in a Petri dish.
PBMCs in the same medium were placed into the upper chamber of each well.
To eliminate evaporation, 8 ml of serum-free culture medium were placed in the bottom of the Petri dish.
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When a porous medium is placed in Loschmidt apparatus, the effective gas diffusion coefficient (EGDC) of this section must be correlated by diffusion coefficient in absence of a porous medium.
In each case 50 mL medium was placed in 250 mL DURAN® baffled flasks.
Two hundred microlitres of YPD medium was placed in each well of the microtiter plate (96 wells) under sterile conditions.
For analysis of Monascus content, 5 ml liquid fermentation medium was placed on four layers of gauze for filtering, and the gauze was then washed with sterile water until the liquid was colorless.
The lower growth rate in our study may have been due to the experimental conditions, in which the vertical photobioreactor containing 20 L of medium was placed outdoors, where there was a low light intensity and the minimum nighttime temperature was 21.5 °C.
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