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Benchtop bioreactor tests with the optimized medium were performed.
Preparing and sterilizing culture medium were performed according to conventional methods [11].
Cultures where no dipeptide was added to the medium were performed parallel to supplemented cultures and considered as reference and named "control".
Repeated cycles of IgG purification from a cell culture medium were performed, each followed by cleaning with aqueous NaOH (0.1 M, 0.5 M and 1 M).
The stability study of graphene oxide and reduced graphene oxide (rGO) in aqueous medium were performed by visual observation and surface charge measurement.
Cell growth assays in PCA medium were performed using C. glutamicum strains F and W, which were grown on 5 mL of BHI medium for 24 h at 30 °C.
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The impact of PtNPs on bacterial growth in liquid medium was performed as follows.
The characterization of the medium was performed using standard instrumental analysis.
The pH control of the medium was performed using a pH meter I-160MI.
Time dependent numerical simulation of relativistic wind interaction with interstellar medium was performed.
A serial transfer of 50 mg of cells (dry weight) into fresh medium was performed every 24 h.
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