Sentence examples for medium were mixed from inspiring English sources

For the preparation of solid media, 10 g of medium were mixed with 25 mL of deionized H2O in a 250 mL Erlenmeyer flask, sealed with a foam plug and tinfoil, and autoclaved.

Fifty µL of serially two-fold diluted peptide in DMEM medium were mixed with 50 µL of JR-CSF and 50 µL of seminal plasma or DMEM.

Isolated human neutrophils suspended in RPMI-1640 medium were mixed with the test compound or vehicle (<0.1% ethanol) and incubated for 5 m.

The next day, cells grown in fresh growth medium were mixed with viral supernatant to a total volume of 2.5 ml for 60 mm-plate and incubated at 37°C in 5% CO2 incubator for one hour.

miRNA mimics (100 pmol) in 200 µl of serum-free, antibiotic-free, medium were mixed with 5 µl of Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA) dissolved in 200 µl of the same medium and allowed to stand at room temperature for 20 min. The resulting 400 µl transfection solutions were then added to each well containing 1.6 ml of medium.

The dissolved β-Lactoglobulin solution and the equilibrated gel medium were mixed for 2 hr at 20°C.

While cells attached, 8 μl of Cellfectin II reagent (Invitrogen) in 100 μl of unsupplemented Grace's medium was mixed with ~2 5 μg of bacmid in 100 μl of unsupplemented Grace's medium and incubated for 15 30 min at 25 °C.

The melted medium was mixed thoroughly with the sample and let them solidify.

The powder of medium was mixed with 1 l of distilled water and the transparent medium was sterilized in 121 °C and then added to the plate.

After which, 10 μl of E. coli suspension in 90 μl PBS medium was mixed with the nanodiamonds chip in PBS buffer.

To quantify PCL-degrading activity, 1 ml of an overnight liquid culture in UF medium was mixed with 20 ml of basal medium containing 0.3% PCL and incubated at 30-50°C 30-50°Ch.