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A549 (2 × 10) or RERF-LC-AI cells suspended in 100 μl medium were instilled into the intraperitoneal cavity of 5-week-old male BALB/c nude mice.
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The contrast medium is instilled using a Foley catheter placed in the rectum just below the anastomosis.
Bacteria (in LB medium diluted 1 1 in PBS) were instilled in the small intestine (duodenum) with a 22 1/2 G needle, in a volume of 1 10 ml.
However, to maintain the amoebic virulence, trophozoites were instilled in mice; seven days later, the brains were recovered in Bacto Casitone medium with antibiotics.
Lung lobes were instilled with approximately 10 mL of 10% neutral buffered formalin (NBF).
Two control mice were instilled with distilled water and treated identically.
(~ 390 μg) sCT/kg were instilled by i.j. or i.c.
Topical proparacaine hydrochloride 0.5% eye drops were instilled preoperatively.
Sized fractions of fibers were instilled intratracheally into Wistar rats.
Five further 25 ml aliquots were instilled and aspirated.
Control groups were instilled with PBS (100 μl) alone.
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CEO of Professional Science Editing for Scientists @ prosciediting.com